Expression of the p16(INK4a) and Ki-67 in relation to the grade of cervical intraepithelial neoplasia and high-risk human papillomavirus infection / 부인종양

OBJECTIVE: The purposes of this study were to evaluate the expression of p16(INK4a) (referred as to p16) and Ki-67 in cervical intraepithelial neoplasia (CIN), and the correlation between high-risk human papillomavirus (HPV) infection and the above biomarkers. METHODS: We analyzed 31 patients who were diagnosed with CIN at Kwandong University Myongji Hospital from October 2006 to September 2007. CIN specimens (CIN1, 12; CIN2, 6; CIN3, 13) were obtained by colposcopy-directed biopsy (CDB) or loop electrical excision procedure (LEEP). The expressions of p16 and Ki-67 were evaluated by immunohistochemical methods with antibodies to p16 and Ki67. The immunohistochemical staining results were classified into four grades: 0, 1, 2 and 3. HPV genotyping or Hybrid Capture-II test was used to detect high-risk HPV. RESULTS: The expression of p16 (p<0.001) and Ki-67 (p=0.003) were positively associated with CIN grade. p16 expressions increased significantly with high-risk HPV infection (p=0.014), especially HPV type 16 and 58. Ki-67 expression was not related with high-risk HPV. There was positive correlation between the expression of the p16 and Ki-67 (p=0.007). CONCLUSION: CIN grade were positively related to the expression of p16 and Ki-67. p16 expressions of high-risk HPV specimens significantly increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV infection, p16 can be a useful biomarker.

The clinical responses of uterine artery embolization to treat uterine leiomyoma for 3 years / 대한산부인과학회지

OBJECTIVE: The aim of our study is to evaluate the effect of uterine artery embolization as primary treatment for symptomatic uterine fibroid METHODS: From December 1999 to December 2004, 25 patients underwent uterine artery embolization for the treatment of uterine fibroid. The patients were followed up at regular intervals (at 1st, 3rd, 6th month and annual) for 3 years. The mean duration of follow-up was about 41 month (range; 41+/-15). During follow-up period, consecutive uterine and fibroid volume, and symptoms were checked. RESULTS: The uterine volumes were significantly reduced by 27% (P=0.0238) after 3month. And the fibroid volumes were significantly reduced by 59% (P=0.0070) after 1month. The uterine volumes were significantly reduced by 27% (P=0.0082) after 3 years. And the fibroid volumes were significantly reduced by 60% (P=0.0445) after 3 years. All women reported noticeable improvement of menorrhagia after complete of follow up. But, two of nine women who had symptoms of dysmenorrhea or lower abdominal pain, showed no changes in symptoms CONCLUSION: Uterine artery embolization appears to be effective in controlling symptoms, and substantially reducing uterine and fibroid volume with few complication after 3 years. However, long term follow-up will be necessary to establish the efficacy of this procedure.